In 1977, Sanger developed a much simpler sequencing method, called “chain termination,” which is today known simply as Sanger sequencing. This technique took advantage of a different type of special nucleotide called a dideoxyribonucleotide, or ddNTP. ddNTPs lack one of the hydroxyl groups present on a normal dNTP, preventing the chemical reaction necessary to add another nucleotide and terminating DNA elongation.
Nature, Published online: 04 March 2026; doi:10.1038/s41586-026-10205-3
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